Ligation of Annealed Oligos into Digested Backbone
3:02
Generation of Cell Lines Stably Expressing spCas9
5:40
Reporter Assay for Cas9 Activity
6:10
Spinfection of sgRNAs into Cas9 Expressing Cells
6:31
Ba/F3 Cellular Transformation and Positive Selection using m-IL3 Withdrawal
7:23
Results: Use of CRISPR/Cas9 Tool and Ba/F3 Cellular Transformation to Study Oncogenic Potential of Mutated Calreticulin
9:02
Conclusion
Transcript
The overall goal of this experiment is to utilize CRISPR/Cas9 gene editing to model calreticulin mutations in cytokine dependent hematopoietic cells in order to study their oncogenic activity. This method can help answer key questions in the hemat
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Målrettet genet redigering med CRISPR/Cas9 har mye lettere forståelsen av biologiske funksjoner gener. Her bruker vi CRISPR/Cas9 metodikken å modell calreticulin mutasjoner i cytokin-avhengige blodkreft cellene for å studere aktiviteten deres kreftfremkallende.