Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
4:45
Bone Marrow Stem Cell (BMSC) Differentiation
6:07
Results: Representative BMSC Differentiation
7:27
Conclusion
Transcript
The overall goal of this procedure is to isolate and culture bone marrow stromal cells, or BMSCs. This method can help answer key questions in the bone and adipocyte fields about progenitor cells. The main advantage of this technique is that BMSCs
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In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts.