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7.6K Views
•
08:39 min
September 16th, 2020
DOI :
10.3791/56800-v
Chapters
0:04
Title
0:36
Mitochondrial Fluorescence Intensity and Cellular ATP Detection
2:45
Mitochondrial Membrane Potential Measurements
4:39
Oxygen Consumption Rate (OCR) Measurements
6:22
Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
7:43
Conclusion
Transcript
这是一个混乱的调用回答一个关键问题,你也有一个领域的功能,如ATP水平和氧气消耗率。新的或一页旧,这种技术是,它很容易执行,它是一个细胞线粒体功能的特殊评估很多。为了开始这个实验,在玻璃底部培养皿中播种2万个 HepG2 细胞。
在37摄氏度的培养箱中加入β-六氯环氧烷或B-HCH,24小时加入5%的二氧化碳。通过将无水DMM细胞培养基中添加无水DMSO,准备一毫摩尔线粒体绿色荧光探针溶液。然后将溶液每盘一毫升添加到玻璃底部培养皿中的细胞中。
并在37摄氏度下孵育4
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Summary
了解环境有机氯农药(OCPs)对肝细胞线粒体功能的影响,对于探讨OCP引起代谢紊乱的机理十分重要。本文提出了检测肝线粒体功能的详细方法。
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