Size Selection for Desired Library Size and Quality Control
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Results: Isolation and High-throughput Sequencing of Poor Quality DNA Isolated from Various Herbarium Species
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Conclusion
Transcript
The overall goal of this protocol is to increase the quantity of isolated double-stranded DNA and subsequent high-throughput sequencing libraries from herbarium specimens, maximizing their utility in phylogenomic studies. This method unlocks the p
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This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.