Growth Conditions to Induce Adventitious Shoots of Ipecac
1:46
Extraction and Purification of Phytohormones
4:46
LC-MS/MS Analysis of IAA and CKs
5:41
Results: IAA and CK Levels in Adventitious Shoots Change During Five Weeks of Culture
7:12
Conclusion
Transcript
The overall goal of this procedure is to describe how endogenous phytohormone levels change during adventitious shoot formation in ipecac. This method can help answer key questions related to tissue regeneration in the plant tissue culture wheel.
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Zufällige Triebe können auf Internodien Segmente der Ipecacuanha ohne Phytohormon Behandlung induziert werden. Um Phytohormon Dynamik während zufällig schießen Bildung zu bewerten, haben wir endogenen Auxin und Cytokinin in Internodien Segmenten von LC-MS/MS gemessen.