Generating Huntingtin-mosaic Transgenic Flies and Adult Brain Dissection
3:39
Whole Brain Fixation, Washing, and Mounting
6:11
Imaging and Quantifying Prion-like Transmission of Aggregates
8:22
Results: Example Images
9:43
Conclusion
Transcript
The overall goal of this procedure is to monitor prion-like spreading of protein aggregates in vivo. Using transgenic flies that express an aggregation prone protein in a soluble version of the same protein in isolated cell populations. This metho
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Akkumulere beviser støtter tanken om, at patogene protein aggregater tilknyttet neurodegenerative sygdomme spredes mellem celler med prion-lignende egenskaber. Her, beskriver vi en metode, der giver mulighed for visualisering af celle til celle spredning af prion-lignende aggregater i model organisme, Drosophila melanogaster.