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DOI :
10.3791/56912-v
•
8:12 min
January 8th, 2018
Chapters
0:05
Title
0:59
Sample Preparation: Cell Culture
2:56
Immunoprecipitation (IP) Assay
7:00
Results: Sample Preparation and IP Enrichment for PTM Detection and Crosstalk Investigation
7:43
Conclusion
研究多, 内源性修饰修改的目标蛋白可能是极具挑战性的。这里描述的技术利用一个优化的裂解缓冲和过滤系统开发了特定的 PTM 靶向, 亲和矩阵, 以检测乙酰化, 泛, sumo 2/3 和酪氨酸磷酸酯的修改为目标蛋白质使用单一的, 流线型的系统。
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