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DOI :
10.3791/56923-v
•
9:11 min
March 26th, 2018
Chapters
0:05
Title
0:47
Fabrication of PDMS Chip
1:51
Fabrication of Patterned Coverslips
3:13
Chip Assembling and Final Implementation
6:03
Neuron Culture and Imaging
7:14
Results: Calibration of Fluorescence eXclusion Method Using a Photoresist Stripe as the Object of Interest and Neuronal Structures Volume Imaging
8:17
Conclusion
容积是细胞生理病理特征的重要参数。我们描述了一种荧光排除方法, 允许对神经生长所隐含的突起和动态结构进行分析所需的亚测微轴向分辨率的体外神经元体积的全场测量。
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