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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

DOI :

10.3791/56969-v

11:27 min

August 28th, 2018

August 28th, 2018

20,695 Views

1Department of Biochemistry, Purdue University

Targeted genome editing in the model system Danio rerio (zebrafish) has been greatly facilitated by the emergence of CRISPR-based approaches. Herein, we describe a streamlined, robust protocol for generation and identification of CRISPR-derived nonsense alleles that incorporates the heteroduplex mobility assay and identification of mutations using next-generation sequencing.

Tags

CRISPR Cas9

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