Una plataforma integrada para la asignación de todo el genoma de cromatina Estados mediante secuenciación del ChIP de alto rendimiento en los tejidos del Tumor
Tissue Lysis, Sonication, and Antibody Preparation
2:52
Chromatin Immunoprecipitation
3:40
Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
4:43
Purification and Quantification of Precipitated DNA
6:00
Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
7:53
Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
9:23
Conclusion
Transcript
The overall goal of this protocol is to unbiasedly identify combinatorial chromatin state patterns in tumor tissues and cancer cell lines. This method could help answer key questions in the cancer epigenetics field such as how a barren histone mod
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Aquí, describimos un protocolo de secuencia de ChIP de alto rendimiento optimizado y tubería de análisis computacional para la determinación de patrones de estado de todo el genoma cromatina de líneas celulares y tejidos del tumor congelado.