Name: simultaneous recordings of cortical local field potentials, electrocardiogram, electromyogram, and breathing rhythm from a freely moving rat
Uploaded: 2018-04-02T13:30:00
Description: Watch this Scientific Journal Video about Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat at JoVE.com

这项工作得到了 Kaken (17H05939; 17H05551)、臣基金会和 Suzuken 纪念基金会的支持。

所有涉及动物的程序都是根据 NIH 的动物保护和使用指南进行的。
1. 集成微驱动器阵列的研制
<ol><li>为皮质 LFP 录制创建微驱动器阵列, 如其他地方所述1,2,3。在电极接口板 (EIB) 上留出至少6个金属孔, 用于心电图/肌电图/BR 通道, 如1.2 所述, 连接到 bioflex 线。</li>
	<li>将 bioflex 线切成6片, 长度为5.0 厘米. 剥?...

<ol>
	<li>Kloosterman, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-drive+Array+for+Chronic+in+vivo+Recording:+Drive+Fabrication.">Micro-drive Array for Chronic in vivo Recording: Drive Fabrication.</a> JoVE. (26), e1094 (2009).</li><li>Nguyen, D. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-drive+Array+for+Chronic+in+vivo+Recording:+Tetrode+Assembly.">Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly.</a> Journal of Visualized Experiments : JoVE. (26), e1098 (2009).</li><li>Jog, M. 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(57), (2011).</li><li>Zeredo, J. L., Kumei, Y., Shibazaki, T., Yoshida, N., Toda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+biting+behavior+induced+by+acute+stress+in+the+rat.">Measuring biting behavior induced by acute stress in the rat.</a> Behav Res Methods. 41 (3), 761-764 (2009).</li><li>Young, G. A., Khazan, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electromyographic+power+spectral+changes+associated+with+the+sleep-awake+cycle+and+with+diazepam+treatment+in+the+rat.">Electromyographic power spectral changes associated with the sleep-awake cycle and with diazepam treatment in the rat.</a> Pharmacol Biochem Be. 19 (4), 715-718 (1983).</li><li>Oishi, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polygraphic+Recording+Procedure+for+Measuring+Sleep+in+Mice.">Polygraphic Recording Procedure for Measuring Sleep in Mice.</a> JoVE. (107), e53678 (2016).</li><li>Chaput, M. 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为了了解大脑如何调节外围活动水平, 反之亦然, 大规模的记录方法同时捕获多体区域的电 biosignals 是必要的。这项研究描述了一个手术程序, 一个记录系统, 监测大脑局部磁场电位, 心率, 肌肉结构的大小和呼吸率, 已经改善了记录系统, 用于脑组织中的细胞外记录。该系统在集成的微驱动器阵列上收集来自大脑和外围器官的电信号到单一的 EIB 上。综合微驱动器阵列的制备应在手术前至少几个小时?...

中枢神经系统控制身体状态以应对各种环境变化, 这种控制通常表现为心率、呼吸率和肌肉收缩的变化。然而, 很少有研究证实了这种外围生理因素与皮质活动有什么关联。为解决这一问题, 有必要从中央和外围组织监测电 biosignals 的大规模记录方法。在大脑皮层 , 局部场电位 ( LFP ) 信号入到皮质组织中的电极记录 extracellularly1,2,3。为了同时记录来自小哺乳动物 (如大鼠和小鼠) 皮质区域的多个 LFP 信号, 许多研究开发出了各种类型的定制电极组件, 称为微驱动器。传统的微驱动器由连接到电极中间部分的金属螺钉 (通常是 tetrodes), 一个容纳螺钉和电极的核心机构, 以及一个可容纳金属孔的电子接口板 (EIB) 来连接电极的打开端 (图 1、图 2和图 3)。这种电极组件使操作者能够控制在数天到数周内插入大脑的许多电极的深度, 并允许进行长期的神经元活动记录, 因为动物受到各种行为任务。在外围器官中, 心跳信号被植入在心脏区域或周围的一对电极上 (心电图), 在4,5,6, 和骨骼肌肉信号被记录作为 electromyograms (EMGs) 与插入到肌肉组织的电极7,8,9。用单单位录制10、11, 研究了嗅球电信号与呼吸 (BR) 节律之间的关系。在传统的记录系统中, 来自不同组织的这些信号被独立的记录装置捕获, 这意味着需要额外的实验系统来精确同步这些多台设备, 同时脑体信号的录音。这一制度是为了克服这一问题而制定的。在该系统中, 所有从外围器官记录的电信号, 包括心电图、EMGs 和反映呼吸节律的嗅球发出的电信号, 都集成到单个微驱动器阵列1,2 ,3, 这里称为一个集成的微驱动器阵列。该系统只需要一个多通道记录设备, 适用于任何常规的微驱动器阵列。这种技术的优点是它不需要任何特殊的设备或触发信号来匹配多个设备的记录时间, 而且它允许更方便的数据处理, 因为所有的信号都被记录为类似的数据类型。这项技术将有助于了解中央和外围器官之间的神经关联。本文介绍了与该技术相关的程序, 并给出了从大鼠获得的代表性数据集。

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			<td height="40" style="height:40px;">EIB-36-PTB</td>
			<td>Neuralynx, Inc., Bozeman, MT</td>
			<td>EIB-36-PTB</td>
			<td>EIB</td>
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			<td>Blackrock&#160; Microsystems, Salt Lake City, UT</td>
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			<td>Blackrock&#160; Microsystems, Salt Lake City, UT</td>
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			<td>Oyaide.com, Tokyo, Japan</td>
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			<td>Narishige, Tokyo, Japan</td>
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			<td>High-speed drill</td>
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			<td height="40" style="height:40px;">Minimo ONE SERIES ver.2</td>
			<td>Minitor Co.,Ltd, Tokyo, Japan</td>
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			<td>High-peed drill Power Supply&#160;</td>
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			<td height="40" style="height:40px;">Provinice 250 mL</td>
			<td>Shofu Inc., Kyoto, Japan</td>
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			<td>DS Pharma Animal Health, Osaka, Japan&#160;</td>
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			<td>Wako Pure Chemical Industries, Ltd., Osaka, Japan</td>
			<td>224-00165&#160;</td>
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			<td>Nacalai tesque, Kyoto, Japan</td>
			<td>31131-85</td>
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			<td height="40" style="height:40px;">Calcium Chloride Dihydrate</td>
			<td>Wako Pure Chemical Industries, Ltd., Osaka, Japan</td>
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			<td height="40" style="height:40px;">Stainless steel screw M1.0&#215;4.0&#160;</td>
			<td>MonotaRO, Hyogo, Japan</td>
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			<td>Stainless steel screw for BR electrodes</td>
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			<td height="40" style="height:40px;width:307px;">Stainless steel screw M1.4&#215;3.0</td>
			<td>MonotaRO, Hyogo, Japan</td>
			<td>42617687</td>
			<td>Stainless steel screw for g/r electrodes and anchors</td>
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simultaneous recordings of cortical local field potentials, electrocardiogram, electromyogram, and breathing rhythm from a freely moving rat

监测大脑和周围组织的生理动态是解决一些问题, 关于大脑如何控制身体功能和内部器官节律当动物暴露在情绪挑战和变化的生活环境。在一般实验中, 来自不同器官的信号, 如大脑和心脏, 是由独立的记录系统记录的, 需要多个记录设备和处理数据文件的不同程序。本研究描述了一种新的方法, 可以同时监测电 biosignals, 包括在多个脑区的局部场电位, 代表心脏节律的心电图, electromyograms 代表清醒/睡眠相关的肌肉收缩, 呼吸信号, 在一个自由移动的老鼠。这种方法的记录配置是基于一个传统的微驱动器阵列, 用于皮层局部场电位记录, 其中有数以万计的电极, 并且从这些电极获得的信号被集成到单个电板安装在动物的头上。在这里, 这个录音系统得到了改进, 使外围器官的信号也被转移到一个电子接口板上。在单个手术中, 电极首先分别植入适当的身体部位和靶脑区。所有这些电极的开端, 然后焊接到在动物的头部以上的电气板的个别通道, 以便所有的信号可以集成到单一的电路板。将此主板连接到记录设备, 可以将所有信号收集到单个设备中, 从而降低了实验成本, 简化了数据处理, 因为所有数据都可以在同一数据文件中处理。这项技术将有助于了解中央和外围器官之间的神经关联。

这种方法可以同时捕获来自多个器官的生物电信号, 这些信号代表大脑的神经元活动, 心率, 呼吸节律和骨骼肌收缩(图 1)。图 4提供了可自由移动的大鼠在矩形框 (25 x 40 厘米2) 中自由觅食的具有代表性的记录数据。示例数据集包括移动和静止状态之间的典型行为转换。用小波分析方法从海马 LFP 跟踪计算出功...

Watch this Scientific Journal Video about Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat at JoVE.com

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

本研究介绍一种自由运动大鼠脑、心电图、electromyograms 和呼吸信号中局部场电位同时记录的方法。这种技术降低了实验成本, 简化了数据分析, 有助于理解大脑和周围器官之间的相互作用。

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

Monitoring the physiological dynamics of the brain and peripheral tissues is necessary for addressing a number of questions about how the brain controls ...

Research

JoVE Journal

Neuroscience

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

科研

神经科学

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

自由移动鸽子穿越三个阶段学习记录单个神经元&#39;动作电位

While the subject of learning has attracted immense interest from both behavioral and neural scientists, only relatively few investigators have observed ...

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

电图同时记录和麻醉大鼠视觉诱发电位

The electroretinogram (ERG) and visual evoked potential (VEP) are commonly used to assess the integrity of the visual pathway. The ERG measures the ...

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

使用新颖的方法在自由移动的新生大鼠中记录脑电图

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such ...

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

急性<em&gt;体内</em&gt;麻醉大鼠超导途径局部场电位和多单位活动的电生理记录

Converging evidence shows that many neuropsychiatric diseases should be understood as disorders of large-scale neuronal networks. To better understand the ...

Noninvasive EEG Recordings from Freely Moving Piglets

自由移动仔猪的无创脑电图记录

The method allows the recording of high-quality electroencephalograms (EEGs) from freely moving piglets directly in the pigpen. We use a one-channel ...

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

自由运动大鼠脑电图记录过程中兴奋氨基酸的微透析

Microdialysis is a well-established neuroscience technique that correlates the changes of neurologically active substances diffusing into the brain ...

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

多纤维光测量记录自由移动动物的神经活动

Recording the activity of a group of neurons in a freely-moving animal is a challenging undertaking. Moreover, as the brain is dissected into smaller and ...

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously

构建局部场势微电极，用于同时从多个脑结构 进行体内 记录

Researchers often need to record local field potentials (LFPs) simultaneously from several brain structures. Recording from multiple desired brain regions ...

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

自由移动小鼠中肠道来源的微生物代谢物的脑室内递送

The impact of gut microbiota and their metabolites on host physiology and behavior has been extensively investigated in this decade. Numerous studies have ...