Preparation of Adherent Platelet and Vascular Cell Monolayers
3:56
Fluorescent Labeling and Perfusion of Leukocytes
6:32
Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
8:09
Conclusion
Transcript
The overall goal of this experiment is to investigate the mechanisms behind the interactions of leukocytes to adherent vascular cells or platelets under flow conditions. This method can help answer key questions in the immunology field such as how
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Leucociti avidamente interagiscono con le cellule vascolari e le piastrine dopo lesione di parete del vaso o durante l'infiammazione. Qui, descriviamo un semplice test a flusso laminare per caratterizzare i meccanismi molecolari che sono alla base delle interazioni tra leucociti ed i loro partner cellulari.