Preparation of Adherent Platelet and Vascular Cell Monolayers
3:56
Fluorescent Labeling and Perfusion of Leukocytes
6:32
Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
8:09
Conclusion
Transcript
The overall goal of this experiment is to investigate the mechanisms behind the interactions of leukocytes to adherent vascular cells or platelets under flow conditions. This method can help answer key questions in the immunology field such as how
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Leucócitos, avidamente, interagir com células vasculares e plaquetas após lesão de parede do vaso ou durante a inflamação. Aqui, descrevemos um simples ensaio baseado em fluxo laminar para caracterizar os mecanismos moleculares que fundamentam as interações entre os leucócitos e os seus parceiros de celulares.