Dissection and Infection of Tonsillar Tissue with HIV-1
4:13
Dissection and Infection of Uterine Cervical Mucosa with HIV-1
7:17
Histoculture
8:33
Results: Kinetics of HIV-1 Replication in Cervical Mucosa Explants
10:02
Conclusion
Transcript
This method can help answer key questions in the HIV field, such as the identification of the determinants of virus transmission and pathogenesis within two of the most effected organs by the infection. In fact, the key advanced driving disease pr
Sign in or start your free trial to access this content
Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.