Results: The Yield of Infectious Baculovirus During Heparin Affinity Chromatography
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Conclusion
Transcript
The overall goal of this protocol is to produce baculovirus in insect cell culture, purify with heparin affinity chromatography and to concentrate with ultracentrifugation. The final product can be used for in vivo gene transfer or as a vaccine. T
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I denne protokol, er baculovirus produceret af forbigående Transfektion af baculovirus plasmid i Sf9 celler og forstærket i et serumfrit suspension kultur. Supernatanten er renset af heparin affinitet kromatografi og yderligere koncentreret af ultracentrifugering. Denne protokol er nyttigt for produktion og oprensning af baculovirus for gen terapi program.