Preparation of Cells for High-resolution Respirometry
3:40
High-resolution Respirometry
10:08
Results: Evaluation of the effects of Monomeric Cocoa Epicatechin or Curcumin on Intact Beta Cell Respiration
11:12
Conclusion
Transcript
The overall goal of this approach is to directly assess the respiratory function of pancreatic beta cells, under insulin stimulatory conditions. This can also be used to observe the effect of different compounds on beta cell respiratory function.
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Målet med denne protokol er at måle effekten af glukose-medieret ændringer til mitokondrie respiration i overværelse af naturlige forbindelser på intakt 832/13 beta celler ved hjælp af høj opløsning respirometri.