Preparation of Cells for High-resolution Respirometry
3:40
High-resolution Respirometry
10:08
Results: Evaluation of the effects of Monomeric Cocoa Epicatechin or Curcumin on Intact Beta Cell Respiration
11:12
Conclusion
Transcript
The overall goal of this approach is to directly assess the respiratory function of pancreatic beta cells, under insulin stimulatory conditions. This can also be used to observe the effect of different compounds on beta cell respiratory function.
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Het doel van dit protocol is voor het meten van het effect van glucose-gemedieerde wijzigingen in mitochondriale ademhaling in de aanwezigheid van natuurlijke verbindingen op intact 832/13 beta cellen met behulp van hoge-resolutie respirometrie.