Preparation of Cells for High-resolution Respirometry
3:40
High-resolution Respirometry
10:08
Results: Evaluation of the effects of Monomeric Cocoa Epicatechin or Curcumin on Intact Beta Cell Respiration
11:12
Conclusion
Transcript
The overall goal of this approach is to directly assess the respiratory function of pancreatic beta cells, under insulin stimulatory conditions. This can also be used to observe the effect of different compounds on beta cell respiratory function.
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Målet med detta protokoll är att mäta effekten av glukos-medierad ändringar av mitokondriell andning i närvaro av naturliga föreningar på intakt 832/13 beta-celler använder högupplösta respiration.