Preparation of Stained DNA and Restriction Enzyme Assay
2:47
Electroeluting Stained DNA in an Agarose Gel
4:44
Results: Digestion of Cyanine Dye-treated Lambda DNA
6:08
Conclusion
Transcript
The overall goal of this experiment is to digest fluorescently labeled DNA molecules with methylation and non-methylation sensitive restriction enzymes and determine the digestion rate of the experiment. This method can help answer key questions i
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Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.