Timed Matings - Collecting, Sorting, and Housing Zebrafish Embryos for Experiments
3:23
Dechorionate, Deyolk, and Fix Zebrafish Embryos
6:09
Staining DNA and FACS Sorting Embryos
8:00
Results: Genome-wide DNA Replication Timing at High-resolution in Zebrafish
9:28
Conclusion
Transcript
The overall goal of this procedure is to generate whole genome DNA replication timing maps from zebrafish embryos in different stages of development. This method can help answer key questions in the cell cycle and chromatin biology fields, such as
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斑马鱼最近被用作一个体内模型系统, 用于研究开发过程中 DNA 复制的时机。这里详细介绍了使用斑马鱼胚胎来描述复制时间的协议。该协议可以很容易地适应于研究突变体、个体细胞类型、疾病模型和其他物种的复制时间。