Timed Matings - Collecting, Sorting, and Housing Zebrafish Embryos for Experiments
3:23
Dechorionate, Deyolk, and Fix Zebrafish Embryos
6:09
Staining DNA and FACS Sorting Embryos
8:00
Results: Genome-wide DNA Replication Timing at High-resolution in Zebrafish
9:28
Conclusion
Transcript
The overall goal of this procedure is to generate whole genome DNA replication timing maps from zebrafish embryos in different stages of development. This method can help answer key questions in the cell cycle and chromatin biology fields, such as
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Zebrafisk blev for nylig brugt som en in vivo modelsystemet for at studere DNA replikation timing under udvikling. Her er detaljerede protokoller for at bruge zebrafisk embryoner til profil replikering timing. Denne protokol kan tilpasses nemt for at studere replikering timing i mutanter, enkelte celletyper, sygdomsmodeller og andre arter.