Timed Matings - Collecting, Sorting, and Housing Zebrafish Embryos for Experiments
3:23
Dechorionate, Deyolk, and Fix Zebrafish Embryos
6:09
Staining DNA and FACS Sorting Embryos
8:00
Results: Genome-wide DNA Replication Timing at High-resolution in Zebrafish
9:28
Conclusion
Transcript
The overall goal of this procedure is to generate whole genome DNA replication timing maps from zebrafish embryos in different stages of development. This method can help answer key questions in the cell cycle and chromatin biology fields, such as
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Zebrafiskar användes nyligen som ett in vivo -modellsystem för att studera DNA-replikering tidpunkten under utveckling. Här är detaljerad protokollen för zebrafiskar embryon profil replikering timing. Detta protokoll kan enkelt anpassas för att studera replikering timing i mutanter, enskilda celltyper, sjukdomsmodeller och andra arter.