Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy (Video) | JoVE
August 2nd, 2018
•1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health
与细胞表面上的靶受体结合的抗体可以赋予构象和聚类改变。这些动态变化对表征靶细胞内药物的发展有影响。该协议利用共焦显微和图像相关光谱学通过 ImageJ/斐济, 以量化的程度, 受体聚类在细胞表面。
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