Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy (Video) | JoVE
August 2nd, 2018
•1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health
Antistoffer som binder å målrette reseptorer på cellens overflate kan gi conformation og klynging endringer. Disse dynamiske endringer har implikasjoner for å karakterisere narkotika utvikling i målcellene. Denne protokollen bruker AC confocal mikroskopi og bilde korrelasjon spektroskopi gjennom ImageJ/FIJI å kvantifisere omfanget av reseptor klynger på cellens overflate.
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