Cell Attachment and Viability with Osteogenic Differentiation
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Results: Osteogenic Differentiation of PDCs and Analysis of 1,25-dihydroxy Vitamin D (Calcitriol) and Vitamin C Treatment of PDCs using RT-PCR
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Conclusion
Transcript
The overall goal of this study is to investigate the osteoinductive effects of Vitamin D on cultured mesenchymal stem cells that have been harvested from the dental alveolar periosteum. The use of mesenchymal stem cells is a great advance in denti
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We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.