Name: generation of size-controlled poly (ethylene glycol) diacrylate droplets via semi-3-dimensional flow focusing microfluidic devices
Uploaded: 2018-07-03T12:30:00
Description: Watch this Scientific Journal Video about Generation of Size-controlled Poly ethylene Glycol Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices at JoVE.com

Dette arbejde blev støttet af Shenzhen grundforskning finansiering (Grant nr. JCYJ 20150630170146829, JCYJ20160531195439665 og JCYJ20160317152359560). Forfatterne vil gerne takke Prof. Y. Chen på Shenzhen Institutes of Advanced Technology, kinesiske Academy of Sciences for understøtter.

1. skimmel fabrikation
<ol><li>Design to komponeneter ved hjælp af en tegning software. Beskrive en skitse af microchannel struktur og bruge to separate lag for maske 1 og 2 i samme tegningsfilen, så sikre, at alle forbindelser mellem forskellige kanaler. Udskrive forskellige lag uafhængigt at krom plade på glas af en kreditor med 1 µm opløsning. Sikre, at komponeneter er mørk med gennemsigtig designet strukturer, som en negativ polaritet. Bemærk: Maske 1 indeholder den spredte fase inlet ka...

<ol>
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Generation af dråber i flow-fokus tilstand ved hjælp af 2D og semi-3D mikrofluid enhed er tidligere blevet udviklet i en række rapporter8,9,15,19,20, 21. I disse systemer, blev den vandige væske, der ikke kunne være størknede valgt som den spredte fase, såsom deioniseret vand8,<sup c...

Droplet-baserede mikrofluid systemer har evnen til at producere meget monodisperse dråber fra nanometer mikrometer diameter vifte1 og holde stort potentiale i høj overførselshastighed drug discovery2, syntese af biomolekyler3 ,4, og den diagnostiske test5. De unikke fordele ved mindre dråber, som de større areal til volumen-forholdet og de store programmer med indtagelse af et par microliters af prøven, har teknologien vakt omfattende interesse i en bred vifte af områder. Emulgering af to ikke-blandbare væsker er en af de mest typiske metoder til at generere slipværktøj. I tidligere rapporter i området, har forskere udviklet en række forskellige droplet dannelse geometrier, herunder t-kryds, strøm-fokusere og co flyder geometrier. I t-kryds geometri, er den spredte fase leveret gennem en vinkelret kanal ind i den vigtigste kanal, hvor den kontinuerlige fase løber6,7. I den typiske todimensionale (2D) strøm-fokusere8,9 geometri, er spredte fase flow forskydes fra lateralt; og for det co strømmende geometri10,11, på den anden side en kapillær at indføre spredte fase flow er placeret, co-aksialt inde i en større kapillær for Co strømmende geometri, så spredte fase flow er forskydes fra alle retninger.
Dråbestørrelse styres ved at justere kanal størrelse og flow rate ratio, og den minimale størrelse produceret af co flyder eller t-kryds er begrænset til snesevis af mikrometer. Strøm-fokusere droplet-dannelse system, tre tilstande af droplet breakup form ved at justere trykket forholdet mellem to faser og overfladeaktivt stof koncentration, herunder dryppende regime, jetting regime og tip-streaming15. Tip-streaming-tilstand kaldes også tråd dannelse og fremkomsten af en tynd tråd tegning fra spidsen af spredte fase flow kegle vil observeres. Tidligere undersøgelser har påvist dråber er mindre end nogle få mikrometer kunne oprettes selvom tip-streaming proces i 2D eller semi-3D strøm-fokusere enhed8,12. Men som en vandig opløsning, som indeholder en meget lav koncentration af PEGDA blev brugt som den spredte fase, svind forholdet mellem PEGDA partikler var omkring 60% af de oprindelige dråber i diameter efter foto-polymerisering, mens PEGDA uden fortynding som den spredte fase førte til ustabile tip-streaming tilstand12. Interfacial spændinger er en vigtig parameter for emulsion proces og det vil falde som følge af tilsætning af det overfladeaktive stof i den kontinuerlige fase væske, fører til fald i Dråbestørrelse, højere generation frekvens13, stærkt buet spids, og at forebygge ustabilitet14. Desuden, når bulk overfladeaktivt stof koncentration er langt højere end den kritiske micelle koncentration, interfacial spændingen er ca uforanderlig i mættede stat13 og tip-streaming-tilstand kan forekomme15.
Baseret på ovenstående bemærkninger i dette papir, udviklet vi en letkøbt tilgang til PEGDA dråber generation ved hjælp af en semi-3D strøm-fokusere mikrofluid enhed, fabrikeret af flere lag blød litografi metode. Forskellig fra den typiske 2D strøm-fokusere enhed, semi-3D strøm-fokusere enheden har en lavvandet spredte fase kanal og en dyb kontinuerlige fase kanal, så den spredte fase kan forskydes fra op og ned ved siden af lateral. Dette giver større tilpasning interval for flow-fokus tilstand ved at reducere den energi og pres der kræves for slipværktøj opløsning. Forskellig fra den forrige rapport12, den spredte fase er ren PEGDAcontaining foto-initiativtager, og sørg for, at krympning forholdet af PEGDA partikler er lavere end 10%16; og den kontinuerlige fase er en blanding af hexadecane opløsning med en høj bulk koncentration af silikone-baserede nonionisk overfladeaktivt stof. Størrelse-styrbar og ensartet dråber blev produceret ved at justere trykket forholdet mellem to faser. Diameteren af dråberne ændres fra 80 µm til 1 µm som droplet bruddet behandler ændringer fra tilstanden jetting tip-streaming-tilstand. Derudover blev PEGDA partikel syntetiseret gennem foto-polymerisering proces under UV-eksponering. Droplet generation mikrofluid system med lethed af fabrikation vil give flere muligheder for biologiske applikationer.

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	<tbody>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">Silicon wafer</td>
			<td style="width:71px;">Huashi Co., Ltd</td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">SU-8 2025, 2100</td>
			<td style="width:71px;">Microchem Co.</td>
			<td style="width:119px;">Y111069</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">SU-8 developer</td>
			<td style="width:71px;">Microchem Co.</td>
			<td style="width:119px;">Y020100</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="91">
			<td height="91" style="height:91px;width:216px;">Chromium mask</td>
			<td style="width:71px;">Qingyi Precision Mask Making Co., Ltd</td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">polydimethylsiloxane(PDMS)</td>
			<td style="width:71px;">Dow Corning</td>
			<td style="width:119px;">Sylgard 184</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">poly(ethylene glycol) diacrylate (PEGDA)</td>
			<td style="width:71px;">Sigma</td>
			<td style="width:119px;">26570-48-9</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">2-hydroxy-40-(2-hydroxyethoxy)-2-methylpropiophenone</td>
			<td style="width:71px;">TCI</td>
			<td style="width:119px;">H1361-5G</td>
			<td style="width:167px;">photoinitiator</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hexadecane</td>
			<td style="width:71px;">Sigma</td>
			<td style="width:119px;">544-76- 3</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">ABIL EM 90</td>
			<td style="width:71px;">CHT</td>
			<td style="width:119px;">144243-53-8</td>
			<td style="width:167px;">surfactants</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Rhodamine B</td>
			<td style="width:71px;">Aladdin</td>
			<td style="width:119px;">81-88-9</td>
			<td style="width:167px;">fluorescent dye</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Spin Coater</td>
			<td style="width:71px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Lithography machine</td>
			<td style="width:71px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Automatic ointment agitator</td>
			<td style="width:71px;">Thinky</td>
			<td style="width:119px;">ARV-310</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Oven</td>
			<td style="width:71px;">BluePard</td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Optical microscope</td>
			<td style="width:71px;">OLYMPUS</td>
			<td style="width:119px;">IX71</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">High-speed camera</td>
			<td style="width:71px;">Hamamatsu, Japan</td>
			<td style="width:119px;">ORCA-flash</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">MAESFLO Microfluidic Fluid Control System</td>
			<td style="width:71px;">FLUIGENT</td>
			<td style="width:119px;">MFCS-EZ</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">UV lamp</td>
			<td style="width:71px;">FUTANSI</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">365 nm UV light, 8000 MW/CM2</td>
		</tr>
	</tbody>
</table>

generation of size-controlled poly (ethylene glycol) diacrylate droplets via semi-3-dimensional flow focusing microfluidic devices

Ensartet og størrelse-styrbar poly (etylenglykol) diacrylate (PEGDA) droplets kunne fremstilles via flow fokusere processen i en mikrofluid enhed. Dette dokument foreslår en semi-three-dimensional (semi-3D) strøm-fokusere mikrofluid chip for slipværktøj dannelse. Polydimethylsiloxan (PDMS) chip var opdigtet benytter metoden multi-lag bløde litografi. Hexadecane som indeholder overfladeaktive stof blev brugt som den kontinuerlige fase, og PEGDA med ultraviolet (UV) foto-initiativtageren var spredte fase. Overfladeaktive stoffer tilladt lokale overfladespænding til at falde og dannet en mere cusped tip, der fremmes, bryde ind i bittesmå mikro-dråber. Som presset af spredte fase var konstant, blev størrelsen af droplets mindre med stigende kontinuerlige fase pres før spredte fase strømmen blev afbrudt. Som et resultat, dråber med størrelse variation fra 1 µm til 80 µm i diameter selektivt kunne opnås ved at ændre trykket forholdet i to indløb kanaler, og den gennemsnitlige variationskoefficient blev anslået til at være under 7%. Derudover kunne droplets forvandle til micro-beads af UV-eksponering for foto-polymerisering. De tråddannende biomolekyler på sådanne micro-beads overfladen har mange potentielle anvendelser inden for biologi og kemi.

Semi-3D strøm-fokusere mikrofluid chip var opdigtet benytter flere lag blød litografi teknikker som beskrevet ovenfor. Den fabrikationsproces og resultater for master mold i protocolare vist i figur 2. Det første lag, som giver en 65 µm bred kanal for at indføre den spredte fase og en 50 µm bred blænde (figur 2en), er 20 µm i tykkelse. En tilføjelse 130 µm tykkelse lag bruges til at give den kontinuerli...

Watch this Scientific Journal Video about Generation of Size-controlled Poly ethylene Glycol Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices at JoVE.com

Generation of Size-controlled Poly ethylene Glycol Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Vi præsenterer her, en protokol for at illustrere de fabrication processer og kontrol eksperimenter af en semi-three-dimensional (semi-3D) strøm-fokusere mikrofluid chip for slipværktøj dannelse.

Generation af størrelse-kontrollerede Poly (etylenglykol) Diacrylate dråber via Semi-3-dimensionelle Flow med fokus mikrofluid enheder

Uniform and size-controllable poly (ethylene glycol) diacrylate (PEGDA) droplets could be produced via the flow focusing process in a microfluidic device. ...

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Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Bestemmelse 3D Flow Fields via Multi-kamera Light Field Imaging

In the field of fluid mechanics, the resolution of computational schemes has outpaced experimental methods and widened the gap between predicted and ...

Thermal Measurement Techniques in Analytical Microfluidic Devices

Termiske Måleteknikker i Analytical mikrofluidenheder

Thermal measurement techniques have been used for many applications such as thermal characterization of materials and chemical reaction detection. ...

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Fabrikation og drift af Acoustofluidic Devices Støtte Bulk Acoustic stående bølger for Sheathless Fokusering af partikler

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must ...

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Oprettelse Sub-50 Nm Nanofluidic vejkryds i PDMS Mikrofluid Chip via Self-Assembly Processen med Kolloid Partikler

Polydimethylsiloxane (PDMS) is the prevailing building material to make microfluidic devices due to its ease of molding and bonding as well as its ...

Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices

Opløsningsmiddel Bonding til Fabrikation af PMMA og COP mikrofluidenheder

Thermoplastic microfluidic devices offer many advantages over those made from silicone elastomers, but bonding procedures must be developed for each ...

Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Scanning Light Scattering Profiler (SLPS) -baseret metode til kvantitativ evaluering af fremadrettet og bagudlysudspredning fra intraokulære linser

The scanning light scattering profiler (SLSP) methodology has been developed for the full-angle quantitative evaluation of forward and backward light ...

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Mikrofluidisk Imaging Flow Cytometry ved asymmetrisk detektion Time-stretch Optical Microscopy (ATOM)

Scaling the number of measurable parameters, which allows for multidimensional data analysis and thus higher-confidence statistical results, has been the ...

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices

Flow-assisteret dielektroforesis: En billig metode til fremstilling af højtydende løsning-bearbejdes Nanotråd enheder

Flow-assisted dielectrophoresis (DEP) is an efficient self-assembly method for the controllable and reproducible positioning, alignment, and selection of ...

Experimental Investigation of the Flow Structure over a Delta Wing Via Flow Visualization Methods

Eksperimentel undersøgelse af strukturen Flow over en Delta Wing Via Flow visualisering metoder

It is well known that the flow field over a delta wing is dominated by a pair of counter rotating leading edge vortices (LEV). However, their mechanism is ...

Safe Experimentation in Optical Levitation of Charged Droplets Using Remote Labs

Sikker eksperimenter i optisk Levitation opladet dråber ved hjælp af Remote Labs

The work presents an experiment that allows the study of many fundamental physical processes, such as photon pressure, diffraction of light or the motion ...