PMC Isolation and Cultivation by Intraperitoneal Lavage
3:14
Fluorometric Intracellular Free Calcium Concentration Measurement in PMCs
6:38
Beta-hexosaminidase Release Measurement in PMCs
8:46
Results: Beta-hexosaminidase Release Colorimetric Multi-well Measurements Performed with PMCs Isolated from Wild Type Mice
10:14
Conclusion
Transcript
These methods can help answer key questions in the field of intracellular signaling on excitable cells, such as which ion channels are critical for immune cell activation and mediator release. The main advantages of these techniques are their rela
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Nous présentons ici un protocole visant à isoler et à cultiver des mastocytes péritonéaux murines. Nous décrivons également deux protocoles pour leur caractérisation fonctionnelle : une imagerie fluorescente de concentration2 + Ca libre intracellulaire et un test de dégranulation issu de dosage colorimétrique de la β-hexosaminidase libéré.