CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

9.9K Views

09:39 min

June 9th, 2018

DOI :10.3791/57311-v

June 9th, 2018

Transcript

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Keywords CRISPR

Chapters in this video

0:04

Title

1:30

In Utero Electroporation

5:16

Preparation of Cryosections from Electroporated Cerebella

6:22

Immunostaining the Cerebellar Cryosections

7:38

Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse

8:48

Conclusion

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