Optimization and Verification of 3' RACE to Map the 3' UTR Using Two Different Enzymes
7:46
Results: Nested Forward Primer Search
8:12
Results: Successful Mapping of Cancer Transcripts
9:03
Conclusion
Transcript
The overall goal of this procedure is to determine the sequences of mRNA transcripts from the three-prime end to regions within the protein coding region by using transcript-specific nested primers in two subsequent PCRs and sequencing the purifie
Sign in or start your free trial to access this content
De to forskellige 3' hurtige forstærkning af cDNA ender (3' RACE) protokoller beskrevet her gøre brug af to forskellige DNA polymeraser til at knytte sekvenser, der omfatter et segment af åben behandling ramme (ORF), stop-codon og den hele 3' UTR af et udskrift ved hjælp af RNA fremstillet af forskellige kræft cellelinjer.