Optimization and Verification of 3' RACE to Map the 3' UTR Using Two Different Enzymes
7:46
Results: Nested Forward Primer Search
8:12
Results: Successful Mapping of Cancer Transcripts
9:03
Conclusion
Transcript
The overall goal of this procedure is to determine the sequences of mRNA transcripts from the three-prime end to regions within the protein coding region by using transcript-specific nested primers in two subsequent PCRs and sequencing the purifie
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