Name: quantification of lipid abundance and evaluation of lipid distribution in caenorhabditis elegans by nile red and oil red o staining
Uploaded: 2018-03-05T14:00:00
Description: Watch this Scientific Journal Video about Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining at JoVE.com

この作品は NIH グラントによって可能になった: R01GM109028 (S.P.C.)

1. ナイル (NR) 脂質の染色
<ol><li>5 mg/mL NR ストック溶液の調製<ol>
<li>500 mL ペットボトルで 100% アセトン 200 mL に NR 粉末 100 mg を追加します。</li>
		<li>光への露出を避けるためにアルミ箔でボトルをカバーします。</li>
		<li>使用前に暗闇の中で 2 時間のためのソリューションをかき混ぜます。</li>
		<li>長期的な使用、光の露出をせず密閉瓶で NR の原液を格納します。研究のニーズに応じて NR 原?...

<ol>
	<li>Greenspan, P., Mayer, E. P., Fowler, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nile+red:+a+selective+fluorescent+stain+for+intracellular+lipid+droplets.">Nile red: a selective fluorescent stain for intracellular lipid droplets.</a> J Cell Biol. 100 (3), 965-973 (1985).</li><li>Guo, Y., Cordes, K. R., Farese, R. V., Walther, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipid+droplets+at+a+glance.">Lipid droplets at a glance.</a> J Cell Sci. 122 (6), 749-752 (2009).</li><li>Horobin, R. W., Kiernan, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Conn's biological stains: a handbook of dyes, stains and fluorochromes for use in biology and medicine. , (2002).</li><li>Hulme, S. E., Whitesides, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemistry+and+the+worm:+Caenorhabditis+elegans+as+a+platform+for+integrating+chemical+and+biological+research.">Chemistry and the worm: Caenorhabditis elegans as a platform for integrating chemical and biological research.</a> Angew Chem Int Edit. 50 (21), 4774-4807 (2011).</li><li>Khanna, A., Johnson, D. L., Curran, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiological+roles+for+mafr-1+in+reproduction+and+lipid+homeostasis.">Physiological roles for mafr-1 in reproduction and lipid homeostasis.</a> Cell Rep. 9 (6), 2180-2191 (2014).</li><li>Lynn, D. A., Dalton, H. M., Sowa, J. N., Wang, M. C., Soukas, A. A., Curran, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Omega-3+and-6+fatty+acids+allocate+somatic+and+germline+lipids+to+ensure+fitness+during+nutrient+and+oxidative+stress+in+Caenorhabditis+elegans.">Omega-3 and-6 fatty acids allocate somatic and germline lipids to ensure fitness during nutrient and oxidative stress in Caenorhabditis elegans.</a> P Natl Acad Sci USA. 112 (50), 15378-15383 (2015).</li><li>Pang, S., Lynn, D. A., Lo, J. Y., Paek, J., Curran, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SKN-1+and+Nrf2+couples+proline+catabolism+with+lipid+metabolism+during+nutrient+deprivation.">SKN-1 and Nrf2 couples proline catabolism with lipid metabolism during nutrient deprivation.</a> Nat Commun. 5, (2014).</li><li>Saltiel, A. R., Kahn, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Insulin+signalling+and+the+regulation+of+glucose+and+lipid+metabolism.">Insulin signalling and the regulation of glucose and lipid metabolism.</a> Nature. 414 (6865), 799-806 (2001).</li><li>Witting, M., Schmitt-Kopplin, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Caenorhabditis+elegans+lipidome:+A+primer+for+lipid+analysis+in+Caenorhabditis+elegans.">The Caenorhabditis elegans lipidome: A primer for lipid analysis in Caenorhabditis elegans.</a> Arch Biochem Biophys. 589, 27-37 (2016).</li><li>Yen, K., Le, T. T., Bansal, A., Narasimhan, S. D., Cheng, J. X., Tissenbaum, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparative+study+of+fat+storage+quantitation+in+nematode+Caenorhabditis+elegans+using+label+and+label-free+methods.">A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods.</a> PloS One. 5 (9), e12810 (2010).</li><li>Zhang, Y., Zou, X., Ding, Y., Wang, H., Wu, X., Liang, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+genomics+and+functional+study+of+lipid+metabolic+genes+in+Caenorhabditis+elegans.">Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans.</a> BMC Genomics. 14 (1), 164 (2013).</li><li>Folick, A., Min, W., Wang, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Label-free+imaging+of+lipid+dynamics+using+Coherent+Anti-stokes+Raman+Scattering+(CARS)+and+Stimulated+Raman+Scattering+(SRS)+microscopy.">Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.</a> Curr Opin Genet Dev. 21 (5), 585-590 (2011).</li><li>Pino, E. C., Webster, C. M., Carr, C. E., Soukas, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biochemical+and+high+throughput+microscopic+assessment+of+fat+mass+in+Caenorhabditis+elegans.">Biochemical and high throughput microscopic assessment of fat mass in Caenorhabditis elegans.</a> J Vis Exp. (73), (2013).</li><li>O'Rourke, E. J., Soukas, A. A., Carr, C. E., Ruvkun, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C.+elegans+major+fats+are+stored+in+vesicles+distinct+from+lysosome-related+organelles.">C. elegans major fats are stored in vesicles distinct from lysosome-related organelles.</a> Cell Metab. 10 (5), 430-435 (2009).</li></ol>

肥満および代謝性疾患の率の上昇は、細胞や組織の脂肪蓄積を調節する機構を研究対象に適したモデルを線虫になります。最近の証拠は、脂質レベルの変化がインスリン シグナル8、ホルモン受容体2、生殖出力5への活性化に至る細胞プロセスに関連付けられることを示唆しています。ラベル無料顕微鏡とクロマト グラフ法、ナイ?...

脂質は、生命に不可欠です。膜の不可欠なコンポーネント、二次メッセンジャーとして機能し信号のトランスデューサー、エネルギー貯蔵に重要な機能を持っています。脂質代謝が調節不全、肥満、II 型糖尿病、公衆衛生の懸念9を押しているような病気に します。線虫(線虫) 比較的短いライフ サイクル、透明体、知られている細胞系統、完全に配列されたゲノムをもっているために、脂質の代謝を研究するためのエクセレント モデル生物であります。両性具有者に主にc. の elegansは、要するに持ち帰り高スループット転送遺伝子スクリーニング遺伝子経路の代謝4の広い配列を勉強する時間の期間の同質の動物の数が多いを高めるために研究者をことができます。このアプローチは、273線虫脂質代謝遺伝子ヒト、マウス、ラット、ショウジョウバエにおける保全の高度を明らかにしました。さらに、線虫の以上 300 の脂質遺伝子人間第オーソロガス メタボリック シンドローム11に関係のない病気に関連付けられているがあります。伝統的に、線虫脂質ストレージの検討が主染料ラベルのアッセイは、脂質蓄積に関する信頼性の高い情報を提供にしていました。あまり一般的は、組織全体で脂質がローカライズして豊富な脂質の測定の違いの説明です。しかし、最近の作品では脂質分布を脂質蓄積6と同じくらい重要にすることができることを明らかにしました。
最近は、高性能液体クロマトグラフィー質量分析 (高速液体クロマトグラフィー-MS)、ガスクロマトグラフィー質量分析法 (GC/MS) などの方法を統合する研究を始めているし、コヒーレント反ストークス アドレスにラマン散乱 (CARS) 顕微鏡、脂質抽出物、特定の脂質画分および脂質堆積物、それぞれ10,11の内容を直接分析することによって汚れ・ ベース ・ アプローチの欠点。さらに、CARS 顕微鏡ではナイルの赤は、脂肪蓄積オフのターゲット自動蛍光細胞小器官10の染色につながる重要な汚れとの使用のため定着性色素として使用する場合のプロキシとして使用できますを明らかにしました。ただし、これらのクロマトグラフィーに関連する必要な技術的な専門知識とコスト、顕微鏡検査方法の使用多くの研究質問理不尽。この記事ではこだわると線虫ナイルの赤を使用して中性脂質沈着汚れし、オイル赤 O の脂質豊富な全動物と特定の組織を区別するために便利で信頼性の高い方法をについて説明します。
ナイルの赤、9-diethylamino-5H-benzo[α]phenoxazine-5-one、benzophenoxazone 染料容易に様々 な有機溶剤に溶解するが、主水に溶解されていません。それは優秀な lysochrome 色素中性脂質トリグリセリドなどを染色するために使用またはコレステロール エステルそれは強い色を特徴とするので可溶性脂質で、周囲の組織とのごくわずかな相互作用よりも溶剤に難溶です。脂質。450-500 と 520 の nm、それぞれ1の励起・発光極大値があります。離散脂質体の腸や他の組織の中でいずれかのクラスターを観察または動物の遺伝子型または実験的な治療によって、均等に分散できる蛍光グリーンのナイルの赤はc. の elegansをステンド グラスを見ると、7。
オイル赤 O、lysochrome、トリグリセリド、リポタンパク質を染色に使用される脂溶性の色素です。アゾ染料は、その化学構造に 3 つの芳香環に接続されている 2 つのアゾ基が含まれているために呼び出されます。それは、イオン化することは困難非常に脂質に溶けるがレンダリングをします。その汚れの色は赤く、その光吸収最大は 518 nm 3。線虫は、オイル赤い O ショー異なったティッシュ6脂質分布の定性的な評価を容易に動物の透明体に対して際立つ赤脂質液滴と汚れます。

<table>
	<tbody>
		<tr>
			<td>Imager.M2m Microscope</td>
			<td>Zeiss</td>
			<td>n/a</td>
			<td>Fluorescence microscope</td>
		</tr>
		<tr>
			<td>ERC5s camera</td>
			<td>Axiocam</td>
			<td>n/a</td>
			<td>Color-capable</td>
		</tr>
		<tr>
			<td>MRm camera</td>
			<td>Axiocam</td>
			<td>n/a</td>
			<td>Fluorescence-capable</td>
		</tr>
		<tr>
			<td>Nile red</td>
			<td>Thermo Fisher</td>
			<td>N1142</td>
			<td>Lipid Stain</td>
		</tr>
		<tr>
			<td>Oil red O</td>
			<td>Alfa Aesar</td>
			<td>A12989</td>
			<td>Lipid Stain</td>
		</tr>
		<tr>
			<td>DAPI</td>
			<td>Thermo Fisher</td>
			<td>D1306</td>
			<td>DNA stain</td>
		</tr>
		<tr>
			<td>Isopropyl Alcohol</td>
			<td>BDH</td>
			<td>BDH1133-1LP</td>
			<td>Fixative solution</td>
		</tr>
		<tr>
			<td>0.2 &#181;m seterile syringe filter</td>
			<td>VWR</td>
			<td>28145-477</td>
			<td>Cellulose acetate filter</td>
		</tr>
		<tr>
			<td>Centrifuge 5430</td>
			<td>Eppendorf</td>
			<td>5428000015</td>
			<td>Centrifuge</td>
		</tr>
		<tr>
			<td>Shaker Rotisserie</td>
			<td>Lab Quake</td>
			<td>400110Q</td>
			<td>Shaker</td>
		</tr>
		<tr>
			<td>Tube Rotator</td>
			<td>VWR</td>
			<td>10136-084</td>
			<td>Rotator</td>
		</tr>
		<tr>
			<td>K2HPO4</td>
			<td>Sigma-Aldrich</td>
			<td>7758-11-4</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>KH2PO4</td>
			<td>Sigma-Aldrich</td>
			<td>7778-77-0</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>MgSO4</td>
			<td>Alfa Aesar</td>
			<td>7786-30-3</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>CaCl2</td>
			<td>Sigma-Aldrich</td>
			<td>10035-04-8</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>NaCl</td>
			<td>Sigma-Aldrich</td>
			<td>7647-14-5</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>Cholesterol</td>
			<td>Sigma-Aldrich</td>
			<td>57-88-5</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>Peptone</td>
			<td>BD Biosciences</td>
			<td>211677</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>Agar</td>
			<td>Teknova</td>
			<td>L9110</td>
			<td>NGM</td>
		</tr>
		<tr>
			<td>LB media</td>
			<td>Sigma-Aldrich</td>
			<td>L3147</td>
			<td>Bacterial growth</td>
		</tr>
	</tbody>
</table>

quantification of lipid abundance and evaluation of lipid distribution in caenorhabditis elegans by nile red and oil red o staining

線虫脂質代謝・ エネルギー代謝を研究するための特別なモデル生物であります。その脂質の遺伝子の多くは人間には維持され、メタボリック シンドロームまたはその他の疾患に関連付けられています。この生物における脂質蓄積の検討は定着性染料またはラベル自由な方法で実施できます。定着性の汚れはナイル川のように赤いとオイル赤 O の脂質レベルを定量的に測定し、質的、組織間でそれぞれ脂質分布を観察する安価な信頼性の高い方法があります。さらに、これらの汚れ各種の脂質代謝遺伝子および細道の高速スクリーニングを可能にします。また、疎水性性質は、脂溶性を容易に、周囲の組織との相互作用を減らす溶媒に解離を防ぐことができます。これらのメソッドは一般的な脂質含量を調べることで効果的なしかし、化学組成と脂質堆積物の多様性の詳細については行いません。これらの目的は、GC-MS と車の顕微鏡などラベル自由な方法がより適していますな、コストにかかわらず。

SKN 1 は bZip 胃酸転写因子 NRF2 の哺乳類と相同を共有して脂肪酸酸化を仲介することが示されています。彼らの食事療法のグルコース濃度に応じて恒常活性skn 1の対立遺伝子を持つワームはナイルの赤7と異なる脂質レベルを表示します。図 1A-C活性skn 1動物脂質レベルを上げることにつながる条件にさら?...

Watch this Scientific Journal Video about Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining at JoVE.com

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

脂質豊かさとナイルの赤とオイル赤 O 染色により線虫の脂質分布評価の定量化

ナイルの赤固定線虫の染色は、組織中の脂質分布の定性的な評価を容易にオイル赤 O 染色中性脂質堆積物の定量的測定法です。

Caenorhabditis elegans is an exceptional model organism in which to study lipid metabolism and energy homeostasis. Many of its lipid genes are conserved ...

The overall goal of these staining methods is to quantify intracellular lipid abundance and evaluate lipid distribution among different tissues. This method can help answer key questions in lipid metabolism such as how are lipids genetically and biochemically regulated and which disease states this regulate lipid homeostasis. The main advantage of this technique is that it is relatively simple and inexpensive to carry out.To carry out Nile Red lipid staining, plate worms on Nematode Growth Medium or NGM seeded with late log OP50 E.coli and grow the worms at 20 degrees Celsius to early L4 stage. Wash the worms with one millimeter of PBST solution and transfer the worm suspension to a 1.5 milliliter microfuge tube. Centrifuge the worms at 560 times gravity for one minute.Then remove the supernatant and repeat the PBST wash until the E.coli is cleared from the suspension. Next, to the worm pellet, add 100 microliters of 40%isopropanol or 60%for ORO staining and incubate the sample at room temperature for three minutes. The addition of isopropanol is crucial for proper permeabilization of the worms before staining.Centrifuge the worms at 560 times gravity for one minute and remove the supernatant without disrupting the worm pellet. It is important to minimize light exposure during the centrifugation steps. Now while in the dark, add 600 microliters of previously prepared NR working solution to each sample.Invert the tubes three times and fully mix the worms and solution. Then incubate the sample in the dark at room temperature for two hours. Following incubation, centrifuge the worms and remove the supernatant.Then add 600 microliters of PBST and incubate the samples in the dark for 30 minutes to remove the excess NR stain. After spinning the samples again as before, remove all but approximately 50 microliters of supernatant and resuspend the worm pellet in the remaining solution. To prepare slides for imaging, pipette five microliters of worm suspension onto a microscope slide and carefully apply a coverslip to avoid trapping any air bubbles.Using the FITC GFP channel to image NR stained worms and trying different exposure times, image the worms at five-fold magnification to capture several animals per field of view. Then switch to 10-fold magnification for better quantification of individual worms. To quantify the images, upload the micrographs to ImageJ.In the image pull down menu, adjust the brightness, contrast of the image deck, and propagate changes to all the images by clicking apply. Use the polygon selection tool to delineate each imaged worm and under the analysis pull down menu, use the measure function to qualify fluorescence intensity emitted by NR.For each image, measure five background locations and calculate the average background fluorescence intensity. Add 600 microliters of Oil Red O or ORO working solution to each sample and invert the tube three times to mix the worms well and the ORO.Rotate the samples at 30 RPM and RT for two hours. Centrifuge the samples at 560 times gravity for one minute and aspirate all the supernatant except for 100 microliters. Then use 600 microliters of PBST to resuspend the samples and rotate the tubes at 30 RPM for 30 minutes to remove excess ORO staining.To better distinguish the location of the animals'germ line in the intestinal cells, stain nuclei by adding one microliter of DAPI for every one milliliter of ORO working solution. Then to prepare slides for imaging, resuspend the worms and the remaining supernatant and mix the solution well. Put five microliters of worm suspension on a microscope slide and carefully apply a coverslip ensuring that no air bubbles form.To image the ORO stained worms, use a color capable camera at five-fold magnification to image several worms in one field of view. Then switch to 10-fold magnification for better examination of individual worms. Finally, after uploading the images to ImageJ and creating an image deck according to the text protocol, categorize the images according to lipid accumulation throughout the animal's body or in specific tissues.SKN-1 shares homology with mammalian Nrf2 and has been shown to mediate fatty acid oxidation. This figure shows activated SKN-1 animals exposed to conditions that lead to increasing lipid levels. As seen in these images, NR fluorescence captured using the FITC GFP channel is prominent along the intestine, but is dimmer in the head, tail, and intestinal lumen.While it is easy to categorize the worms according to the presence or absence of Age-dependent Somatic Depletion of Fat or ASDF, it may be difficult to identify animals with intermediate fat loss. Compared to non-ASDF animals, which show bright red staining throughout the body with few translucent areas, ASDF worms exhibit noticeable fat depletion in intestinal cells. Animals in the process of developing ASDF often show translucent spots where most of the fat loss eventually occur.Moreover, ASDF worms may still feature bright red staining in the head and tail regions because the phenotype is not defined by complete somatic fat loss, but by extensive fat depletion relative to non-aged animals. Once mastered, this technique can be done in three hours plus imaging time if it is performed properly. While attempting this procedure, it's important to remember to wash and permeabilize worms no more than 30 minutes before staining.Following this procedure, other methods like CARS microscopy can be performed to compare the type of lipid species each technology identifies and to determine which method is better suited for specific types of questions. After watching this video, you should have a good understanding of how to quantify lipids and evaluate lipid distribution in the worms using Nile Red and Oil Red O staining, followed by ImageJ micrograph analysis.

Research

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Biology

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

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電離放射線は、次のγH2AXの空間分布の評価

An early molecular response to DNA double-strand breaks (DSBs) is phosphorylation of the Ser-139 residue within the terminal SQEY motif of the histone ...

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Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

細胞外のフラックスを用いた Explanted 網膜組織のエネルギー代謝の測定

High acuity vision is a heavily energy-consuming process, and the retina has developed several unique adaptations to precisely meet such demands while ...

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

ショウジョウバエ幼虫における大鼻細胞の解剖と脂質液滴染色

Lipids are essential for animal development and physiological homeostasis. Dysregulation of lipid metabolism results in various developmental defects and ...

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

成人心臓線維芽細胞と筋線維芽細胞の分離と特徴付け

Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that ...

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

刺激されたラマン散乱顕微鏡を用いた カエノラム型エレガンス における脂質蓄積ダイナミクスの無標識イメージング

Lipid metabolism is a fundamental physiological process necessary for cellular and organism health. Dysregulation of lipid metabolism often elicits ...

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

Caenorhabditis elegansにおける凝集定量の自動化

A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

カエノラブディティス・エレガンスにおける長寿と遺伝子転写解析のための脂質補給

Aging is a complex process characterized by progressive physiological changes resulting from both environmental and genetic contributions. Lipids are ...

Oil Red OとBODIPYを用いた脂質液滴サイズのライブセルイメージングと融合

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

著者スポットライト:ウシ肝細胞における脂肪滴サイズと融合の評価  

ウシ肝細胞における脂肪滴サイズと融合の評価

Lipid droplets (LDs) are organelles that play an important role in lipid metabolism and neutral lipid storage in cells. They are associated with a variety ...