Day One: Infection of K562 Cells with DENV in the Presence of ZIKV Convalescent
3:53
Day 3: Fixation and Flow Cytometry
4:51
Data Analysis
5:31
Results: Antibody-dependent Enhancement of Dengue Virus
6:31
Conclusion
Transcript
The overall goal of this procedure is to determine the capacity of sera collected from Zika virus infected rhesus macaques to mediate antibody dependent enhancement of dengue virus infection in vitro. This method can help answer key questions rela
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We describe a simple and easy protocol for measuring antibody dependent enhancement of infection by Zika virus convalescent serum using Dengue virus Reporter Viral Particles.