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JoVE Journal

Developmental Biology

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Kromozom segregasyon mitoz sırasında canlı hücre görüntüleme

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06:39 min

March 14th, 2018

March 14th, 2018

9,125 Views

0:04

Title

1:04

Labeling Cells

2:21

Visualization of Lagging Chromosomes

4:45

Results: Nocodazole-treated MEFs Lacking PP2A-B56 Undergo Chromosomal Mis-segregation

5:29

Conclusion

Transcript

The overall goal of this method is to label and visualize live chromosomes in mitotic cells using Histone 2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system. This method can be used to visualize mitotic chromosomes in cultured

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Bu iletişim kuralı etiket ve canlı kromozomlar Mitotik hücre Histone2B-GFP BacMam 2.0 etiket ve bir iplik kaset confocal mikroskobu sistemi kullanarak görselleştirmek için basit ve uygun bir yöntem açıklanır.

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