Results: Spatially-resolved Quantification of Ethambutol in Pulmonary Lesions by LCM-LC/MS
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Conclusion
Transcript
The overall goal of this analytical technique is to quantify the anti-tuberculosis drug ethambutol within tuberculosis pulmonary lesion tissue compartments to determine whether bactericidal drug concentrations are being reached. This method can an
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Here, we describe a protocol using laser capture microdissection coupled with LC/MS analysis to spatially-quantify drug distributions within pulmonary tuberculosis granulomas. The approach has broad applicability to quantifying drug concentrations within tissues at high spatial detail.