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DOI :
10.3791/57413-v
•
11:58 min
March 5th, 2018
Chapters
0:04
Title
1:18
Generation of DNA-protein Crosslink Containing Plasmid
6:21
Transfection into Mammalian Cells
8:39
SSPE-qPCR
10:02
Results: Use of Strand-specific Primer Extension Based q-PCR to Quantify the DNA-protein Crosslinks
11:08
Conclusion
本协议的目的是量化在质粒 dna 上对定义的 dna 蛋白 crosslinks 的修复。损伤质粒被转染成受体哺乳动物细胞系和低分子量, 在多时间点后再染。DNA 修复动力学的量化使用链特定引伸后, qPCR。
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