Establishing Human-derived 3D Gastric Fundic Organoids
5:23
Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
7:44
Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
9:12
Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
10:53
Conclusion
Transcript
The overall goal of this culture system is to generate a primary mouse or human-derived gastric monolayer for the study of regeneration in a wound healing assay. This method can help answer key questions in the gastric field such as the mechanism
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在这里, 我们描述了建立和培养人和老鼠衍生的3维 (3D) 胃 organoids 的协议, 以及将 3D organoids 转移到2维单层的方法。本文还介绍了用胃上皮单层作为再生研究的一种新的擦伤创面检测方法。