Establishing Human-derived 3D Gastric Fundic Organoids
5:23
Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
7:44
Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
9:12
Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
10:53
Conclusion
Transcript
The overall goal of this culture system is to generate a primary mouse or human-derived gastric monolayer for the study of regeneration in a wound healing assay. This method can help answer key questions in the gastric field such as the mechanism
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Her beskriver vi en protokol til oprettelse og dyrkning menneskelige - og mus-afledte 3-dimensionelle (3D) gastrisk organoids, og metoden for overførslen af 3D organoids til en 2-dimensional éncellelag. Brug af gastrisk epitelial éncellelag som en roman scratch-sår assay for regenerering undersøgelser er også beskrevet.