Establishing Human-derived 3D Gastric Fundic Organoids
5:23
Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
7:44
Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
9:12
Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
10:53
Conclusion
Transcript
The overall goal of this culture system is to generate a primary mouse or human-derived gastric monolayer for the study of regeneration in a wound healing assay. This method can help answer key questions in the gastric field such as the mechanism
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Her beskriver vi en protokoll for å etablere og dyrking menneske - og mus-avledet 3-dimensjonale (3D) gastrisk organoids og metoden for overføring av 3D organoids til en 2-dimensjonal monolayer. Bruk av mage epithelial monolayer som en roman scratch-sår analysen for regenerering studier er også beskrevet.