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JoVE Journal

Neuroscience

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Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

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07:25 min

March 27th, 2018

March 27th, 2018

8,468 Views

0:04

Title

1:00

Preparation of Fresh Frozen Brain Sections

2:35

RNA In Situ Hybridization Fluorescent Multiplex Assay

5:12

Results: Drd1α RNA Level in Nucleus Accumbens Region of F344/N Rats

6:35

Conclusion

Transcript

The overall goal of this novel RNA in-situ hybridization assay is to visualize single RNA molecules in a specific brain area which are critical for clarifying dopaminergic dysfunction during central nervous system disease. This method can help ans

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Identification of dopamine D1-alpha receptor in the nucleus accumbens is critical for clarifying D1 receptor dysfunction during a central nervous system disease. We performed a novel RNA in situ hybridization assay to visualize single RNA molecules in a specific brain area.

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