Integration Strand Transfer Assay, Fraction Selection, and Storage
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Results: Isolation and Evaluation of PFV Intasomes
9:45
Conclusion
Transcript
The overall goal of this procedure is to assemble and purify prototype foamy virus intasomes which may be then be used for biochemical, structural and kinetic studies. This method can help answer key questions in the retrovirology field such as th
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Integrasa de retrovirus recombinante y oligómeros de ADN mímico extremos de DNA virales pueden formar un complejo enzimático activo conocido como un intasome. Intasomes puede ser usada para estudios bioquímicos, estructurales y cinéticos. Este protocolo detalla cómo montar y purificar el prototipo virus espumoso de los intasomes.