Results: Representative Inflammasome Activation and Pyroptotic Cell Death Detection
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Conclusion
Transcript
The overall goals of this protocol are to examine inflammasome activation at the single cell level via fluorescence microscopy and to measure lysis during pyroptosis by a lactate dehydrogenase, or LDH release assay. This method can help answer key
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We describe the detection of NLRP3 inflammasome activation on cellular basis using fluorescence microscopy and staining for active caspase-1 and the adaptor, ASC. A lactate dehydrogenase release assay is presented to detect pyroptotic lysis on a population basis. These techniques can be adapted to study many aspects of inflammasome biology.