Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
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Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
6:31
Conclusion
Transcript
The overall goal of this protocol is to visualize step-wise procedures involved in the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with chemical and physical agents of interest. Thi
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Se describe un protocolo detallado para el ensayo de mutación Lambda seleccione cII en cultivos de células de roedores transgénicos o los correspondientes animales tratados con un agente químico físico de interés. Este enfoque ha sido ampliamente utilizado para las pruebas de mutagenicidad de agentes carcinógenos en células de mamíferos.