Preparation of Potassium Ion-selective Microelectrodes
4:18
Calibration of Potassium Ion-selective Microelectrodes
5:40
Acute Slice Preparation
7:16
Measurement of Electrically Evoked K Release
9:26
Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K Release
10:53
Conclusion
Transcript
The overall goal of this technique is to make, calibrate, and use monopolar potassium ion-selective microelectrodes. Using such electrodes enables the quantitative assessment of evoked potassium ion concentration dynamics in adult brain slices. Th
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Kalium ioner bidrage til hvile membranen potentiale celler og ekstracellulære K+ koncentration er en afgørende regulator af cellulære ophidselse. Vi beskriver hvordan man laver, kalibrere og bruge monopolære K+-selektive microelectrodes. Ved hjælp af sådanne elektroder kan måling af elektrisk evoked K+ koncentration dynamics i voksen hippocampus skiver.