빠른 고 손쉬운 파이프라인 C. 선 충 을 사용 하 여 CRISPR/Cas9 Ribonucleoproteins에서 게놈 포인트 돌연변이 생성에 대 한

0:42

sgRNA Target Selection and Homology-directed Repair Template Design

2:21

Prepare Injection Mix and Injection Protocol

3:18

Screen P0 Plates and Single mCherry(+) F1s

4:03

Single Worm PCR and Genotyping

5:41

Indentification and Sequence Verification of Edited Animals

6:34

Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1

7:50

Conclusion