sgRNA Target Selection and Homology-directed Repair Template Design
2:21
Prepare Injection Mix and Injection Protocol
3:18
Screen P0 Plates and Single mCherry(+) F1s
4:03
Single Worm PCR and Genotyping
5:41
Indentification and Sequence Verification of Edited Animals
6:34
Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1
7:50
Conclusion
Aqui, apresentamos um método para engenheiro do genoma de c. elegans usando CRISPR-Cas9 ribonucleoproteínas e modelos de reparo dependente de homologia.