Uma rápida e superficial Pipeline para gerar Genomic ponto mutantes em c. elegans usando CRISPR/Cas9 ribonucleoproteínas

0:42

sgRNA Target Selection and Homology-directed Repair Template Design

2:21

Prepare Injection Mix and Injection Protocol

3:18

Screen P0 Plates and Single mCherry(+) F1s

4:03

Single Worm PCR and Genotyping

5:41

Indentification and Sequence Verification of Edited Animals

6:34

Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1

7:50

Conclusion