Sample Collection and Preparation for Histological Staining and Slides
5:09
Hematoxin & Eosin Staining
6:07
Periodic Acid-Schiff Staining
7:19
Sample Collection and Preparation for RNA Extraction
8:30
Results: Histological Analysis of C. albicans Infection in Mice
10:00
Conclusion
Transcript
The overall goal of this procedure is to observe the activation of the immune system during skin infections by injecting Candida albicans to preserve skin architecture and the localization of specific immune and non-immune cells. This method can h
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Nous décrivons ici un protocole qui permet l’analyse histologique et moléculaire des échantillons de peau après injection intradermique de Candida albicans . Ce protocole permet la localisation des cellules immunitaires tissulaires-résident ou nouvellement recrutés, mais aussi la distribution de l’agent pathogène et maintient l’intégrité structurale de la peau.