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JoVE Journal

Immunology and Infection

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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

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10:45 min

June 13th, 2018

June 13th, 2018

10,561 Views

0:05

Title

0:54

C. albicans Preparation

2:29

Deep Dermal Injection

3:57

Sample Collection and Preparation for Histological Staining and Slides

5:09

Hematoxin & Eosin Staining

6:07

Periodic Acid-Schiff Staining

7:19

Sample Collection and Preparation for RNA Extraction

8:30

Results: Histological Analysis of C. albicans Infection in Mice

10:00

Conclusion

Transcript

The overall goal of this procedure is to observe the activation of the immune system during skin infections by injecting Candida albicans to preserve skin architecture and the localization of specific immune and non-immune cells. This method can h

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Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.

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