Sample Collection and Preparation for Histological Staining and Slides
5:09
Hematoxin & Eosin Staining
6:07
Periodic Acid-Schiff Staining
7:19
Sample Collection and Preparation for RNA Extraction
8:30
Results: Histological Analysis of C. albicans Infection in Mice
10:00
Conclusion
Transcript
The overall goal of this procedure is to observe the activation of the immune system during skin infections by injecting Candida albicans to preserve skin architecture and the localization of specific immune and non-immune cells. This method can h
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Her beskriver vi en protokoll som tillater histologiske og molekylære analyse av hud prøver etter Candida albicans intradermal injeksjon. Denne protokollen opprettholder den strukturelle integriteten til huden og gir lokaliseringen av vev-bosatt eller nylig rekruttert immunceller samt patogen fordelingen.