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Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

DOI :

10.3791/57641-v

March 27th, 2019

March 27th, 2019

5,888 Views

1Center for iPS Cell Research and Application (CiRA), Kyoto University

Here, we present a detailed protocol to differentiate human pluripotent stem cells (hPSCs) into pancreas/duodenum homeobox protein 1+ (PDX1+) cells for the generation of pancreatic lineages based on the non-colony type monolayer growth of dissociated single cells. This method is suitable for producing homogenous hPSC-derived cells, genetic manipulation and screening.

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Pancreas Duodenum Homeobox Protein 1

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