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DOI :
10.3791/57651-v
June 13th, 2018
Chapters
0:04
Title
0:53
Preparation of Neutralized Collagen
1:55
Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
4:08
Image Acquisition Using Light-sheet Microscopy
5:07
Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
7:17
Results: Visualization of Human CTL Cells by Light-sheet Microscopy
8:32
Conclusion
在这里, 我们提出了一个协议, 以可视化的免疫细胞嵌入在三维 (3D) 胶原基质中使用光片显微镜。该协议还阐述了如何在3D 中跟踪单元迁移。该协议可用于3D 矩阵中其他类型的悬浮单元。
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